Stat5 reporter assay

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August undefined, 2024

WebHygromycin resistance selectable marker means choosing transient transfection or generating a stable cell line Choose a response element ISRE SBE SIE STAT5 RE Size 20µg Catalog number selected: E4041 $ 435.00 Your price: Log in Add to Cart Add to Helix Cytokine Signaling Pathway Analysis Response Element Firefly Luciferase Vectors … WebIn an attempt to use the cells for large-scale chemical screens to identify JAK3 inhibitors, we established a cell line, 32D/IL-2Rβ/6xSTAT5, stably expressing a STAT5 reporter gene. Treatment of this cell line with IL-2 or IL-3 dramatically increased the reporter activity in a high-throughput format.

Cytokine Signaling Pathway Analysis Response Element Firefly …

WebAssay Entry Cell-based terbium phospho-specific activity (stable) Cell State Dividing Cells Detection Method Fluorescent Gene Symbol STAT5A Optimal Response GM-CSF (EC50 = … WebOct 1, 2004 · IL-4/GFP reporter mice ... This suggests that STAT5 may be involved in asthma through mast cell pathogenesis (8,26) and there have been several ... Enzyme‐linked … hide searching

Modulation of STAT signaling by STAT-interacting proteins

WebReadily assessable STAT5-inducible SEAP reporter activity The stability for 20 passages has been verified Functionally tested and guaranteed mycoplasma-free Applications of HEK-Blue™ IL-9 cells: Detection of human IL-9 (100 pg/ml - 100 ng/ml) and murine IL-9 (30 pg/ml -100 ng/ml) Screening of anti-IL-9 and anti-IL-9R antibodies References: 1. WebMay 12, 2010 · A skewed ratio of pro-inflammatory to anti-inflammatory cytokines, elevated growth factor synthesis and T- and B-lymphocyte activation are 3 hallmarks of rheumatoid arthritis (RA) pathology. Interleukin-6 (IL-6), IL-7, IL-17, IL-12/IL-23 and growth factors, granulocyte macrophage-colony stimulating factor, IL-3, and erythropoietin activate the … WebMar 19, 2013 · Reporter assay to monitor oncogenic FLT3-ITD activity. As FLT3-ITD strongly activates STAT5, 3, 4, 12, 13, 20, 24, 26 we employed a FLT3-ITD-driven STAT5 activation reporter assay for the screen ... how far am i from asheville nc

STAT5 is a key transcription factor for IL-3-mediated ... - Nature

Development of a high-throughput cell-based reporter assay for ...

WebThis assay utilized the growth factor-dependent human erythroleukemia TF1 cell line engineered to express a green fluorescent protein-STAT5 fusion protein. Furthermore, a complementary beta-lactamase reporter gene assay was developed to analyze the transcriptional activity of STAT5 downstream of JAK2 in TF1 cells. WebJan 30, 2024 · We describe here the development and validation of a reporter gene assay (RGA), based on an IL-5-dependent TF-1 cell line variant we established that stably … how far am i from barton college how far am i from birmingham alabama

"WebMay 25, 2000 · It is clear, however, that Stat5 tetramer formation is not critical for Stat5-mediated transcription of reporter constructs containing high affinity GAS motifs (John et al., 1999), a finding that ... " - Stat5 reporter assay

Stat5 reporter assay

A cellular reporter assay to monitor insulin receptor kinase activity ...

Web6.2 STAT5 Signaling. STAT5 was shown to be a mediator of erythropoietin-induced neuroprotection in a rat model of global cerebral ischemia (Zhang, Wang, Cao, Gao, & … WebSTAT5 reporter assay services include screening of STAT5 agonists/antagonists, EC50/IC50 evaluation of those molecules, and functional analysis of STAT5 signaling …

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WebMay 1, 2011 · One day after transfection, β-galactosidase reporter assays were performed. Specific activities from cells transfected with p ... Stat5 reporter gene expression in tissue sections from liver, kidney, spleen, thymus, and uterus. Indicated organs were isolated from C57BL/6 wild-type mice (row 1) and Stat5-REIN reporter mice (rows 2 and 3 ... WebA highly sensitive method for determination of insulin receptor (IR) kinase activity in whole cells, which is based on a STAT5 (signal transducer and activator of transcription 5)-dependent reporter gene assay, has been developed.

WebMar 11, 2024 · There is evidence from cyclin D1-luciferase reporter assays that STAT5 binds at -481 bp of the cyclin D1 promoter . Oct-1 has also been shown to have a role in the transcriptional regulation of cyclin D1. Oct-1 (Octamer-Binding Protein 1) is a transcription factor ubiquitously expressed in the nucleus that contains a POU ... WebFeb 2, 2024 · STAT5 is a key member of the JAK/STAT core cancer pathway, activated by a plethora of cytokines and growth factors to regulate a wide spectrum of physiologic …

WebMay 25, 2000 · The finding that glucocorticoid receptor (GR) is associated with Stat5 came from studies on the β-casein gene. Upon interaction with its steroid ligand, GR binds to specific DNA sequence, the... WebThe STAT5 Reporter (Luc)-Ba/F3 cell line is designed for monitoring STAT5 signal transduction pathways. It contains a firefly luciferase gene driven by the STAT5 response … The One-Step™ Luciferase Assay System is designed for high-throughput, sensitive … Recombinant Sortase A, His-tag (BPS Bioscience #71086) dose-dependent …

WebAssay Interferences Interference testing should be relevant to the patient population that will be tested with a given assay endogenous substances - highest reported clinically relevant …

WebSTAT5 reporter system is designed to monitor the STAT5 transcriptional activity. STAT5 reporter assay services include screening of STAT5 agonists/antagonists, EC50/IC50 evaluation of those molecules, and functional analysis of STAT5 signaling pathways. Assay results will contain a written report and downloadable Excel- or Prism-based data. hide section breaks in wordWebDec 1, 1999 · A highly sensitive method for determination of insulin receptor (IR) kinase activity in whole cells, which is based on a STAT5 (signal transducer and activator of transcription 5)-dependent reporter gene assay, has been developed. We show in Rat1 fibroblasts stably overexpressing the human IR (Rat1- … hide section breaks in word onlineWebReporter Vector primer 4 (RVprimer4) binding region 4171–4190 Sequence information for the pGL4 Vectors is available online at: www.promega.com/vectors/ Example Protocol In … hide section in servicenowWebNov 17, 2016 · Here, we aimed to delineate adipocyte STAT5 functions in the context of lipid metabolism in white adipose tissue. We generated an adipocyte specific knockout of … hide section in wordpressWebFeb 2, 2024 · Two duplicate plates were set up, one for SEAP reporter assay and one for CellTiter-Glo cell viability assay. SEAP was determined using QUANTI-Blue (InvivoGen, rep-qbs). hide selected cells in excelWebMoreover, bioinformatics analyses and luciferase reporter assay indicated that exosomal miR-182-5p was bound to PPARG 3' UTR sequence and inhibited the expression of PPARG. Collectively, our findings provided a new role of EPCs-EXO in the clinical treatment of diabetic skin wounds. ... (STAT5) to control EPC cell-cycle progression 44. In ... hide seek map fortnite codesWebAug 3, 2016 · STAT5 deficiency prevented IL-3-mediated inhibition of osteoclastogenesis, suggesting a key role of STAT5 in IL-3-mediated inhibition of osteoclast differentiation. In … hide section in word