Phospho tbk1 antibody

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August undefined, 2024

WebDec 9, 2013 · TBK1 is phosphorylated in its activation loop, leading to IRF3/7 activation and Type I interferon (IFN) production. IPS-1 and STING localize to the mitochondria and endoplasmic reticulum,... WebDec 10, 2015 · Immunofluorescence experiments using a phospho-TBK1 (pS-172) specific antibody on A549, H1650, Calu-6 and PC9 non-small cell lung cancer (NSCLC) cell lines as well as the immortalized human tracheobronchial epithelial cell line AALE established that phospho-TBK1 localized to centrosomal regions during prophase and prometaphase, …

Tank binding kinase 1 is a centrosome-associated kinase ... - Nature

WebRecombinant Anti-NAK/TBK1 (phospho S172) antibody [EPR2867 (2)] - BSA and Azide free (ab186469) Datasheet. Certificate of Compliance. Submit a review Submit a question … WebPhospho-TBK1/NAK-S172 Rabbit mAb (AP1026) Western blot analysis of extracts of various cell lines, using Phospho-TBK1/NAK-S172 Rabbit mAb (AP1026) at 1:1000 dilution.Both HeLa cells and NIH/3T3 cells were … the park avenue bank

(PDF) Cell Type-Specific Subcellular Localization of Phospho-TBK1 …

WebRecombinant Anti-NAK/TBK1 (phospho S172) antibody [EPR2867 (2)] - BSA and Azide free (ab186469) Datasheet Certificate of Compliance Submit a review Submit a question References (1) $905 Product size 100 µg $905 1 mg $8,985.00 Add to basket Order now and get it on Thursday March 02, 2024 Custom quote Bulk request Shipping info WebTBK1 [p Ser172] Antibody (EPR2867 (2)) Summary. Immunogen. A phospho specific peptide corresponding to residues surrounding serine 172 of human NAK/TBK1 was used … the park ave diner

Phospho-TBK1 (Ser172) Polyclonal Antibody - Thermo …

Anti-TBK1 Antibodies Invitrogen - Thermo Fisher Scientific

WebProduct Details Antibody Details Format Details Citations & References Brand: BD Phosflow™ Alternative Name: T2K, NAK Reactivity: Human (QC Testing) Isotype: Mouse IgG1, κ Immunogen: Human TBK1 C terminus Recombinant Protein Application: Intracellular staining (flow cytometry) (Routinely Tested) Vol. Per Test: 20 µl RRID: AB_1645537 WebSerine/threonine-protein kinase TBK1, NF-kappa-B-activating kinase, T2K, TANK-binding kinase 1, TBK1, NAK: Target/Specificity : This TBK Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S172 of human TBK. Dilution : WB~~1:1000 DB~~1:500: Format the park avenue dietWebDec 1, 2024 · AMPK directly phosphorylates TBK1 at S511 to prime antiviral sensing • AMPK-TBK1 couples glucose deficiency sensing and innate immune surveillance • Targeting the AMPK-TBK1 axis compromises innate antiviral immunity Summary Nutrient sensing and damage sensing are two fundamental processes in living organisms. shuttle report

"WebWhile TBK1 is known to phosphorylate cargo receptors to promote ubiquitin binding, it is unknown whether TBK1 phosphorylates other proteins to promote mitophagy. Using global quantitative proteomics, we identified S72 in RAB7A, a RAB previously linked with mitophagy, as a dynamic target of TBK1 upon mitochondrial depolarization. " - Phospho tbk1 antibody

Phospho tbk1 antibody

Phospho-TBK1 (Ser172) Antibody Affinity Biosciences

WebOur TBK1 polyclonal, monoclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Rabbit, Mouse and Goat. These antibodies have been verified by Knockdown, Knockout and Relative expression to confirm specificity to TBK1. Find the TBK1 antibody that fits your needs. WebWestern blot - Phospho-TBK1/NAK-S172 Rabbit pAb (AP0847) Western blot analysis of HeLa, using Phospho-TBK1/NAK-S172 antibody (AP0847) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit …

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WebTBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a … WebThe Phospho-Specific Mouse Anti-EGF Receptor pSer1047 ( VMA00746) and anti-total Rabbit Anti-EGF Receptor were incubated together. hFAB Rhodamine Anti-Tubulin was added with the fluorescent secondary antibodies. The fluorescent images were captured using the ChemiDoc MP Imaging System. 5 Phosphatase treatment

WebPhospho-TBK1/NAK (Ser172) (D52C2) XP ® Rabbit mAb (PE Conjugate) recognizes endogenous levels of TBK1 only when phosphorylated at Ser172. This antibody may … WebWestern blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight) followed by treatment with LPS (1 μg/ml), up to 24h, using Phospho-TBK1/NAK (Ser172) (D52C2) XP ® Rabbit mAb (upper), or total TBK1/NAK (D1B4) Rabbit mAb #3504 (lower). Show More

WebHere we show that STING stimulates IRF3 phosphorylation by the kinase TBK1 in an in vitro reconstitution system. Using this system, we identified a carboxyl terminal region of STING that is both necessary and sufficient to activate TBK1 and stimulate IRF3 phosphorylation. WebThe Phospho-TBK1 (Ser172) assay measures TBK1 when phosphorylated at Ser172. Contrary to Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis or transfer. The Phospho-TBK1 (Ser172) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor.

WebJan 30, 2024 · TBK1 Antibody (A-6) is an IgG 1 κ mouse monoclonal TBK1 antibody (also designated NAK antibody, T2K antibody, Encephalopathy, acute, infection-induced, herpes-specific, 8 (IIAE8) antibody, or Frontotemporal dementia and/or amyotrophic lateral sclerosis 4 (FTDALS4) antibody) suitable for the detection of the TBK1 protein of mouse, rat and …

WebJul 7, 2024 · Colocalization of DNA with ubiquitin and phospho-TBK1 was also defective in Gt and ∆CTT cells but was normal in ... Cells were stained with the following antibodies: APC CD11b (Biolegend ... the park ave north hotel new yorkWebJan 29, 2015 · Specification of TBK1-mediated IRF3 activation is essential for the tight regulation of IFN production, which would otherwise lead to autoimmune diseases. … the park ave club florham park njWebTBK1 (TANK-binding kinase 1)/NAK (NF-κB activating kinase) is an IκB kinase (IKK)-activating kinase and can activate IKK through direct … the park avenue club florham park njWebJan 17, 2024 · TBK1 Antibody (108A429) is an IgG1 κ mouse monoclonal TBK1 antibody (also designated TBK1 antibody or TANK binding kinase 1 antibody) suitable for the detection of TBK1 of mouse, rat and human origin by WB, IP and ELISA. ... Phospho-enriched whole cell lysates and tissue extracts are available for phospho-specific antibody positive … the park avenue armory nycWebImmunogen: Phosphorylated Human TBK1 Application: Intracellular staining (flow cytometry) (Routinely Tested) Vol. Per Test: 20 µl RRID: AB_647214 Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% … the park ave north hotel upper east sideWebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: … the park avenue armory conservancyWebThis product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free production For … the park ave north hotel nyc