Ph-ncas9-ppe-v2
Webb24 mars 2024 · pH-ePPE: a plant nCas9-RT and pegRNA expression vector that includes an OsU3 promoter-driven SpCas9-based sgRNA scaffold and a ZmUbi promoter-driven … WebbFor sgRNA expression in monocotyledons protoplasts. Loading... 170131. pH-nCas9-PPE-V2. For plant prime editing in rice plants or monocotyledons protoplasts. Loading...
Ph-ncas9-ppe-v2
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WebbTotal vector size (bp) 6388 Vector type Plant Expression, CRISPR Growth in Bacteria Bacterial Resistance (s) Spectinomycin, 50 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number Unknown Gene/Insert Gene/Insert name BsaI:AATG:Cas9_2:GCTT:BsaI Species Synthetic Insert Size (bp) 4141 Tag / Fusion Protein WebbpH-nCas9-PPE-V2 Sequences (1) Addgene Sequences: Full (1) Full Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated …
Webb24 juli 2024 · Product Name : pH-nCas9-PPE-V2 article : bacterial resistance : Kanamycin cloning : backbone pHUE411 (modified from pCambia) backbone_mutation … WebbpH-nCas9-PPE-V2 Map uploaded by the depositor. Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn about the latest plasmid technologies and research tools. Subscribe. Contact Addgene. Have questions about ...
http://www.plantgenomeediting.net/ WebbPrime editing (PE) applications are limited by low editing efficiency. Here we show that designing prime binding sites with a melting temperature of 30 °C leads to optimal performance in rice and that using two prime editing guide (peg) RNAs in trans encoding the same edits substantially enhances PE efficiency. Together, these approaches boost …
http://enpcce.genetics.cas.cn/PN/CXG/ACXG/
WebbPlasmid nCas9-PPE from Dr. Caixia Gao's lab contains the insert nCas9(H840A)-M-MLV and is published in Nat Biotechnol. 2024 May;38(5):582-585. doi: 10.1038/s41587-020 … ipaintwithneedleWebb21 feb. 2024 · Cas9 protein (1 μg) and sgRNA (1 μg) were mixed with the purified target DNA (100–150 ng) in Cas9 reaction buffer (20 mM HEPES, pH 7.5, 150 mM KCl, 10 mM MgCl 2, 0.5 mM DTT) in a total volume of 20 μl, followed by digestion at 37 °C for 0.5–1 h. ipaint wrapsWebbpOsU3 pTaU3 pH-nCas9-PPE-V2/pH-ePPE Forward primer (5'-3'): + Spacer sequence + Reverse primer (5'-3'): + PBS and RT template sequence + Submit >> clear. Help … openshift ssh to nodeWebbpH-nCas9-PPE-V2 pSpG-PPE pH-ePPE ePPE pRN120 Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn … ipa investment advisorsWebb22 juni 2024 · Answer. The dCas9, also known as endonuclease deficient Cas9, is a mutant form of Cas9, whose endonuclease activity is removed by mutating the endonuclease … ipaint sydneyWebb24 mars 2024 · Engineered RTs, fusing functional elements or peptides to nCas9-RT (Cas9 nickase-reverse transcriptase), increasing the binding affinity of the nuclear localization signal sequences of nCas9... openshift stretch cluster latencyWebb24 mars 2024 · We used the ePPE to generate rice plants tolerant to sulfonylurea and imidazolinone herbicides, observing an editing frequency of 11.3% compared with 2.1% … i paint that