WebThe potential of getting a significant number of false positives (FPs) in peptide-spectrum matches (PSMs) obtained by proteomic database search has been well-recognized. … WebJul 16, 2024 · Here are five of the key challenges they identified: 1. Pushing for high-throughput and commercialization. "One of the trends that is occurring in the field is people trying to come up with ways to be more efficient and more high-throughput. One of the complaints from funding agencies is that you can sequence literally thousands of …
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WebPhosphoproteomics is a branch of proteomics that identifies, catalogs, and characterizes proteins containing a phosphate group as a posttranslational modification. … WebAug 4, 2016 · An encouraging recent trend is the validation of quantitative FFPE proteomics findings using orthogonal methods such as immunohistochemistry and immunoblotting [Citation 3, Citation 4]. A study by Ostasiewicz et al. [ Citation 5 ] combined quantitative proteomic analysis of FFPE colorectal carcinoma specimens with messenger … WebFormalin-fixed, paraffin-embedded (FFPE), biobanked tissue samples offer an invaluable resource for clinical and biomarker research. Here, we developed a pressure cycling technology (PCT)-SWATH mass spectrometry workflow to analyze FFPE tissue proteomes and applied it to the stratification of prostate cancer (PCa) and diffuse large B-cell … chromatine functie